| 名称
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Guanylin, rat, mouse
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| 编码
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| 别名
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Guanylin, rat, mouse
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| 纯度
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80%，90%，95%，98%，99%
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| 重量
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1mg,5mg,10mg,50mg,100mg,1g
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| 序列（单字母缩写）
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PNTCEICAYAACTGC (C4&C12 bridge , C7&C15 bridge)
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| 序列（三字母缩写）
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Pro-Asn-Thr-Cys-Glu-Ile-Cys-Ala-Tyr-Ala-Ala-Cys-Thr-Gly-Cys(Cys4&Cys12 bridge , Cys7&Cys15 bridge)
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| 基本描述
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A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.
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| 溶解度
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| 分子量
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1515.7
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| 化学式
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C60H90N16O22S4
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| 存储条件
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Store at -20°C. Keep tightly closed. Store in a cool dry place.
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| 注释
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| Documents
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| Figures
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| Reference
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R.C. Wiegand et al., BBRC, 185, 812 (1992)
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| C端
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| N端
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| 化学桥
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(Cys4&Cys12 bridge , Cys7&Cys15 bridge)
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