| 名称
|
Uroguanylin, human
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| 编码
|
[154525-25-4]
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| 别名
|
Uroguanylin, human
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| 纯度
|
80%，90%，95%，98%，99%
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| 重量
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1mg,5mg,10mg,50mg,100mg,1g
|
| 序列（单字母缩写）
|
NDDCELCVNVACTGCL (C4&C12 bridge, C7&C15 bridge)
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| 序列（三字母缩写）
|
H-Asn-Asp-Asp-Cys-Glu-Leu-Cys-Val-Asn-Val-Ala-Cys-Thr-Gly-Cys-Leu-OH (trifluoroacetate salt)(Cys4 and 12 bridge, Cys7 and 15 bridge)
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| 基本描述
|
A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.
|
| 溶解度
|
|
| 分子量
|
1667.9
|
| 化学式
|
C64H102N18O26S4
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| 存储条件
|
Store at -20°C. Keep tightly closed. Store in a cool dry place.
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| 注释
|
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| Documents
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| Figures
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| Reference
|
T. Kita et al., Am. J. Physiol., 266, F342 (1994)
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| C端
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| N端
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| 化学桥
|
(Cys4 and 12 bridge, Cys7 and 15 bridge)
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